Description: A method for detecting CSFV kit comprising RNA inhibitor, AMV (avian myeloblastosis leukemia virus) reverse transcriptase, Taq polymerase (DNA polymerase, thermal), water-free RNA enzyme, One ? Step RNA PCR (polymerase chain reaction step RNA) mixture, positive and negative controls as well as the sequence SEQ ID NO:???? 1 to SEQ ID NO:?? a mixture of six primer. In the detection process of the present invention will be reverse transcription and PCR amplification in the reaction step is completed, so that the detection time from 4-5 hours to 2-3 hours of the current, thereby facilitating rapid detection.Meanwhile, the invention uses three pairs of primers, so that the PCR method compared with the conventional method has a higher sensitivity to help prevention and implicit CSFV infected animals removed, to avoid causing a wide range of infections. The method is applicable to any laboratory and primary prevention and control institutions at all levels, veterinary stations and large and small farms and the like.
DOI: C12Q1 / 70 (2006.01) I; C12Q1 / 68 (2006.01) I